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Splits emission into two separate channels

Simultaneous acquisition of two emission channels

Simultaneous acquisition of up to four emission channels

Related Notes:

Endocytosis with Multicolor TIRFM

FRET Imaging

Notes by Category:
Spectral Imaging Notes Multichannel Notes

Spectral Imaging Systems

Multichannel imaging systems


Ratiometric Calcium Imaging

Indo-1 is a UV-excitable ratiometric indicator that is used to study the role of calcium in cellular regulation. Ratiometric indicators have significant advantages over single-emission probes (i.e., probes for which changes in ion concentration cause changes in emission intensity only at a single wavelength).

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